The Journal of Phytopharmacology 2024; 13(1):1-3 DOI:10.31254/phyto.2024.13101

Research Article

Antiproliferative effect of methanolic extract of Mallotus philippensis in MCF-7 cell lines

Alfin Mariya¹ , Nisaath Begum² , Arya Mohan³ , Sujith Samraj⁴ , Nisha AR⁵

Alfin Mariya,Nisaath Begum,Arya Mohan,Sujith Samraj,Nisha AR

1. MSc Scholar, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur 680651, India
2. Ph D scholar, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680651, India
3. Teaching Assistant, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur 680651, India
4. Assistant Professor, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Kerala 680651, India
5. Associate Professor and Head, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur 680651, India

*Author to whom correspondence should be addressed.

Received: 1st October, 2023 / Accepted: 15th December, 2023 / Published : 28th February, 2024

Abstract

Phytochemicals and their derivatives are promising options for improving cancer treatment efficiency while minimising side effects. Methanolic extract of flowers of Mallotus philippensis were assessed for their cytotoxicity in MCF-7cell line by 3-(4,5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay at concentrations of 640, 320, 160, 80, 40, 20, 10 and 5 µg/mL and the half maximal inhibitory concentration (IC₅₀) was calculated using Graph Pad Prism 5.0. As a positive control, doxorubicin was used. Phytochemical analysis of M. philippensis methanolic extract using standard tests revealed the presence of alkaloids, tannins, flavonoids, and phenolic compounds. When the cells were exposed to different concentrations of the extracts, a dose-dependent reduction in cell viability was observed. The IC₅₀ of M. philippensis methanolic extract is 41.28 µg/mL. The antiproliferative effect of methanolic extract of M. philippensis in cancer cells in a dose dependent manner could be a promising strategy in chemotherapy. This extrapolates the use of natural products in drug designing conducting clinical trials in vivo and in human subjects.